Production of Cellulase and Xylanase Using an Amazon Fungus (Trametes sp.)

Rafael Lopes e Oliveira; Salony Aquino Pereira; Sergio Duvoisin Junior; Leonor Alves de Oliveira da Silva; Albuquerque

Rafael Lopes e Oliveira Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil and Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.
Salony Aquino Pereira Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Sergio Duvoisin Junior Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Leonor Alves de Oliveira da Silva Laboratory of Fermentative Processes, Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, CEP 50670-901, Recife-PE, Brazil.
Patr�cia Melchionna Albuquerque Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil and Graduate Program in Biotechnology and Natural Resources, School of Health Sciences, State University of Amazonas, CEP 69065-001, Manaus-AM, Brazil.

Keywords: Cellulolytic activity, xylanolytic activity, Trametes sp., experimental design

Abstract

In order to use the residue from the beneficiation of Brazil nuts (Bertholletia excelsa HBK) as substrate in solid-sate fermentation (SSF), in this work, the production of cellulase and xylanase by the fungus Trametes sp. was investigated, using the residue as a carbon source. Employing a 2^4-1 fractional experimental design, the influence of substrate moisture, nutrient addition and inoculum quantity on enzymatic activities was verified. Moisture was detected to be statistically significant for the production of both enzymes, and increasing the moisture leads to the improvement of cellulase and xylanase activities. Nitrogen and phosphate were also important for enzymes production by the Amazon Trametes sp. The use of this Amazon strain to obtain cellulase and xylanase via SSF of Brazil nut residue appears to be feasible when maintaining substrate moisture at 80%, nitrogen source at 0.9% and low inoculum concentrations.