Evaluation of Toxicity on Mice and Artemicidal Activity of Bauhinia monandra Leaf Lectin (BmoLL)
Theory and Applications of Microbiology and Biotechnology Vol. 1,
Page 43-52
Abstract
Aims: This work aimed to determine the toxicity of Bauhinia monandra leaf lectin (BmoLL) to mice and Artemia salina.
Study design: BmoLL was purified from the leaves of B. monandra through affinity chromatography and tested against mice and A. salina in order to evaluate its toxicity.
Methodology: The lectin purification was performed through saline precipitation and chromatography onto a guar gel column. The acute toxicity of BmoLL was evaluated using female mice during 14 days. Toxicity of BmoLL to A. salina larvae was also evaluated.
Results: BmoLL did not induce death or weight loss of mice at 2000 mg/kg b.w.; additionally, the lectin did not affect the survival of A. salina at the assayed concentrations (250–1,000 µg/mL).
Conclusion: BmoLL is an interesting molecule to investigate pharmacological applications due to its low toxicity according to the two models used in this work.
Keywords:
- Acute toxicity
- hemagglutinin
- Bauhinia monandra leaf lectin
- risk assessment
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