Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media

  • Hidetaka Noritomi Department of Applied Chemistry for Environment, Tokyo Metropolitan University, Tokyo, 192-0397, Japan.
Keywords: Adsorption, biochar, ?-chymotrypsin, catalysis, solvent effect

Abstract

The adsorption immobilization of the serine protease ?-chymotrypsin (?-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improved the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed ?-CT. The transesterification rate of BCP-adsorbed ?-CT was much superior to that of free ?-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed ?-CT was about 760 times higher than that of free ?-CT in n-butyl acetate.

 

Published
2020-01-16
How to Cite
Noritomi, H. (2020). Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media. New Advances in Materials Science and Engineering Vol. 2, 124-132. Retrieved from https://stm1.bookpi.org/index.php/namse-v2/article/view/894
Issue
New Advances in Materials Science and Engineering Vol. 2
Section
Chapters