Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media
New Advances in Materials Science and Engineering Vol. 2,
Page 124-132
Abstract
The adsorption immobilization of the serine protease α-chymotrypsin (α-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improved the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed α-CT. The transesterification rate of BCP-adsorbed α-CT was much superior to that of free α-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed α-CT was about 760 times higher than that of free α-CT in n-butyl acetate.
Keywords:
- Adsorption
- biochar
- α-chymotrypsin
- catalysis
- solvent effect
How to Cite
Noritomi, H. (2020). Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media. New Advances in Materials Science and Engineering Vol. 2, 124-132. Retrieved from https://stm1.bookpi.org/index.php/namse-v2/article/view/894
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