Calcite Type Fired Shell Powder of Bivalve Corbicula japonica Improved Mal-Functions of Liver in Animal and in Calcite-Ingested Clients

Sasaki Jin-Ichi; Minegishi Yoshinori; Someya Tetsunei

Sasaki Jin-Ichi Hirosaki University of Health and Welfare, Junior College, 2-5 Ogimachi Ogimachi Hirosaki 036-8104, Japan.
Minegishi Yoshinori Fuji Clean-Tec Co. Ltd. 2-19-21, Tomita Aomori 038-0004, Japan.
Someya Tetsunei Neo Circle Co. Ltd. 8-8-4, Akasaka Minato-Ku Tokyo 107-0052, Japan.

Keywords: Multi-functional calcite, fired shell powder of Corbicula japonica calcite, liver function improvement, NK cells activation, cytokine, liver cells proliferation, lowering lipid level

Abstract

It has been inherited for a long preriod in a small village that the fired shell powder of bivalve Corbicula japonica has been working against liver disorder without any scientific evidences. Experiment was initiated by the village office requirement to ascertain an anti-hepatitis activity of the fired shell powder of the Corbicula japonica. Shell of the Corbicula japonica was fired to analyze a crystal structure by the X-ray diffraction apparatus, followed by animal tests by hepatitis model Long Evans Cinnamon (LEC) rat. In C57BL/6 mouse model additional biofunctions like immune activation, lipid controlling, anti-alcoholic damage activity and liver cells proliferation tests were evaluated. The fired shell powder of the Corbicula japonica (calcite type) fired at 500C for 2 hrs demonstrated the liver function improving activity in LEC rat model via orally administerationt. The calcite type shell powder fed-LEC rats elongated survival times by the improving acute hepatitis symptoms, however, the Corbicula japonica aragonite and Ruditapes philippinarum calcite did not work effectively. Further Corbicula japonica calcite lowered AST(glutamic oxaloacetic transaminase) ALT (glutamic pyruvic transaminase), TBiI (total bilirubin) and IAP (immunosuppressive acidic protein) value in serum, and enforced cellular immune by increasing NK (natural killer) cells activity. Cytokines generation as TNF-? (tumor necrosis factor), IL-2 (interleukin) and IFN-? (interferon) in spleen cells cultivation assay was not clearly admitted. In the alcohol-fed mouse test, the Corbicula japonica calcite reduced alcoholic caused damages in liver and lowered lipid level in serum.